Supramolecular displacement-mediated activation of a silent fluorescence probe for label-free ligand screening |
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Authors: | Torres Diego Amado Azagarsamy Malar A Thayumanavan S |
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Institution: | Department of Chemistry, University of Massachusetts at Amherst, Amherst, Massachusetts 01003, USA. |
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Abstract: | We report a new approach for the rapid screening of analyte binding affinities for a target protein. We demonstrate that a molecular probe, with a pro-fluorophore substrate and ligand moieties, can be hindered from enzymatic access when bound to the target protein. When analytes displace the probe from the protein's binding pocket, a fluorescence profile is generated. This profile is used to discriminate analytes based on their relative binding affinities. |
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