SEQUENCE SPECIFICITY OF TETRAHYDROBENZOPSORALEN PHOTOBINDING TO DNA |
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| Authors: | S Mobilio L Tondelli M Capobianco O Gia |
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| Institution: | Department of Pharmaceutical Sciences of Padua University, Via Marzolo 5, 35131 Padova, Italy;CNR, Istituto dei Composti del Carbonio Contenenti Eteroatomi e loro Applicazioni, Via P. Gobetti 101, 40129 Bologna, Italy |
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| Abstract: | Abstract— The sequence specificity of photobinding to DNA of two tetrahydrobenzopsoralen derivatives has been investigated by testing the photoreactivity toward a number of self-complementary oligonucleotides. The thermodynamic constant for noncovalent binding to each DNA sequence was evaluated. The extent of photoreactivity was greatly dependent upon base composition. The two tetracyclic compounds show similar behavior in comparison to other bifunctional derivatives. Their overall rate constants were greatly enhanced in comparison to classical psoralens. However, their high efficiency of covalent binding is counterbalanced by low affinity for noncovalent interaction with DNA. |
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