Analysis of melatonin using a pH- and temperature-responsive aqueous chromatography system |
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| Authors: | Ayano Eri Suzuki Yusuke Kanezawa Masahito Sakamoto Chikako Morita-Murase Yuko Nagata Yoshiko Kanazawa Hideko Kikuchi Akihiko Okano Teruo |
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| Affiliation: | Department of Physical Pharmaceutical Chemistry, Kyoritsu University of Pharmacy, Tokyo, Japan. |
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| Abstract: | A new method for the qualitative and quantitative analysis of an intracerebral hormone, such as melatonin, has been proposed, utilizing newly designed copolymers that include ion-exchange groups. These copolymers responded to both the temperature and the pH, and the copolymers were modified with cross-linked hydrogel applied onto aminopropyl silica beads. The products were evaluated as HPLC packing materials for a pH- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in both the temperature and the pH. In the chromatographic system, we investigated how to change the retention of melatonin by varying the temperature. A pH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules. |
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