Simple method for determining nucleobase pK(a) values by indirect labeling and demonstration of a pK(a) of neutrality in dsDNA |
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| Authors: | Moody Ellen M Brown Trevor S Bevilacqua Philip C |
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| Institution: | Department of Chemistry, The Pennsylvania State University, University Park, PA 16802, USA. |
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| Abstract: | Phosphorothioates were substituted into double-stranded DNA to study protonated Class I A+.C basepairs by 31P NMR. The method was effective in reporting the A+.C pKa. pKa values near 7.0 were found with optimal nearest-neighbor partners. Such pKa values could expand the catalytic repertoire of nucleic acids. |
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