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The Anticancer Activity and HSA Binding Properties of the Structurally Related Platinum (II) Complexes
Authors:Reza Yousefi  Sadaf Aghevlian  Fatemeh Mokhtari  Hamidreza Samouei  Mehdi Rashidi  S Masoud Nabavizadeh  Zohreh Tavaf  Zahra Pouryasin  Ali Niazi  Reza Faghihi  Mohammad Mehdi Papari
Institution:1. Protein Chemistry Laboratory (PCL), Department of Biology, College of Sciences, Shiraz University, Shiraz, 71454, Iran
5. Institute of Biotechnology, Shiraz University, Shiraz, Iran
2. Department of Mechanical Engineering, College of Engineering, Shiraz University, Shiraz, Iran
4. Department of Chemistry, Shiraz University of Technology, Shiraz, Iran
3. Department of Chemistry, College of Sciences, Shiraz University, Shiraz, 71454, Iran
6. Nuclear Engineering department and Radiation Research center, College of Engineering, Shiraz University, Shiraz, Iran
Abstract:The development of resistance and unwanted harmful interaction with other biomolecules instead of DNA are the major drawbacks for application of platinum (Pt) complexes in cancer chemotherapy. To conquer these problems, much works have been done so far to discover innovative Pt complexes. The objective of the current study was to evaluate the anti cancer activities of a series of four and five-coordinated Pt(II) complexes, having deprotonated 2-phenyl pyridine (abbreviated as C^N), biphosphine moieties, i.e., dppm?=?bis(diphenylphosphino) methane (Ph2PCH2PPh2) and dppa?=?bis(diphenylphosphino)amine (Ph2PNHPPh2), as the non-leaving carrier groups. The growth inhibitory effect of the Pt complexes Pt(C^N)(dppm)]PF6: C 1 , Pt(C^N)(dppa)]PF6: C 2 , and Pt(C^N)I(dppa)]: C 3 , toward the cancer cell lines was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. In addition, the florescence quenching experiments of the interaction between human serum albumin (HSA) and the Pt complexes were performed in order to obtain the binding parameters and to evaluate the denaturing properties of these complexes upon binding to the general carrier protein of blood stream. The structure?Cactivity relationship studies reveal that four-coordinated Pt complexes C 1 and C 2 with both significant hydrophobic and charge characteristics, not only exhibit strong antiproliferation activity toward the cancer cell lines, but also they display lower denaturing effect against carrier protein HSA. On the other hand, five-coordinated C 3 complex with the unusual intermolecular NH??Pt hydrogen binding and the intrinsic ability for oligomerization, exhibits poor anticancer activity and strong denaturing property. The current study reveals that the balance between charge and hydrophobicity of the Pt complexes, also their hydrogen binding abilities and coordination mode are important for their anticancer activities. Moreover, this study may suggest C 1 and C 2 as the potential template structures for synthesis of new generation of four-coordinated Pt complexes with strong anticancer activities and weak denaturing effects against proteins.
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