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Bromelain Enzyme from Pineapple: In Vitro Activity Study under Different Micropropagation Conditions |
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| Authors: | Jaci Lima Vilanova Neta Ana da Silva Lédo Aloisio André Bonfim Lima José Carlos Curvelo Santana Nadjma Souza Leite Denise Santos Ruzene Daniel Pereira Silva Roberto Rodrigues de Souza |
| Affiliation: | 1. Federal University of Sergipe, Av. Marechal Rondon s/n, 49100-000, Aracaju, SE, Brazil 2. Embrapa Tabuleiros Costeiros, CP 44, 49025-040, Aracaju, SE, Brazil 3. University Nove de Julho, Rua Vergueiro 235, 1504-001, S?o Paulo, SP, Brazil 4. ITP??Institute of Technology and Research, Tiradentes University Campus Farolandia, 49032-490, Aracaju, SE, Brazil 5. IBB??Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Campus Gualtar, 4710-057, Braga, Portugal |
| Abstract: | The aim of this work was to evaluate the activity of bromelain in pineapple plants (Ananas comosus var. Comosus), Pérola cultivar, produced in vitro in different culture conditions. This enzyme, besides its pharmacological effects, is also employed in food industries, such as breweries and meat processing. In this work, the enzymatic activity was evaluated in the tissues of leaves and stems of plants grown in culture medium without plant growth regulator. The most significant levels of bromelain were observed in leaf tissue after 4?months of culture in vitro in medium with a filter paper bridge, followed by medium gelled by the agar. The results of this study, regarding the different structures of the pineapple (leaves and stems) in vitro showed that the activity of bromelain varied depending on the culture conditions, the time and structure of which was quantified, ensuring a viable strategy in the production of seedlings with high levels of bromelain in subsequent phases of micropropagation. |
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