Simultaneous GC-MS quantitation of o-phosphoric, aliphatic and aromatic carboxylic acids, proline, hydroxymethylfurfurol and sugars as their TMS derivatives: In honeys |
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Authors: | K Horváth I Molnár-Perl |
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Institution: | 1. Institute of Inorganic & Analytical Chemistry, L. E?tv?s University, P.O. Box 32, 1518, Budapest 112, Hungary
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Abstract: | Summary A GC-MS procedure is described for the simultaneous quantitatation of the minor and major constituents of honeys, as their
trimethylsilyl derivatives, from one solution, by one injection. Selected minor components (aliphatic and aromatic carboxylic
acids, members of various homologous series, together with o-phosphoric acid, proline and hydroxymethylfurfurol), have been
determined on the basis of their characteristic fragment ions, in the presence of extremely high excess of honeysaccharides.
Selective fragmentation of these minor compounds in the ion trap detector provided possibilities for distinguishing them.
The method permitted the simultaneous quantitation of o-phosphoric, malic, shikimic, citric/isocitric, quinic, margaric, oleic
and stearic acids, hydroxymethylfurfurol and proline with the extremely high sugar contents of honeys (fructose, glucose,
galacturonic acid, inositol, sucrose, trehalose, turanose, maltose, gentiobiose, isomaltose, raffinose, erlose, melezitose,
maltotriose, panose, isomaltotriose) and allowed the fast evaluation of sugar and acid constituents of fifteen honeys from
various floral and geological origin. Results revealed that (i) the minor components varied in the concentration range of
0.0001 to 0.43%, and, (ii) together with the saccharides of honeys made up the total of identified and determined constituents
from 87.8% to 98.5%. Quantitative evaluation of the minor constituents was performed on the basis of their selective fragment
ion values with an average reproducibility of 6.7% (RSD).
Presented at: Balaton Symposium on High-Performance Separation Methods, Siófok, Hungary, September 3–5, 1997 |
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Keywords: | Gas chromatography-mass spectrometry Honeys Carboxylic acids 5-Hydroxymethylfurfurol Proline |
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