| 寡聚脱氧核苷酸吸附状态随电位的变化 |
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| 引用本文： | 沈鹤柏,周文骏,杨海峰,余沛涛. 寡聚脱氧核苷酸吸附状态随电位的变化[J]. 物理化学学报, 2002, 18(6): 490-494. DOI: 10.3866/PKU.WHXB20020603 |
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| 作者姓名： | 沈鹤柏 周文骏 杨海峰 余沛涛 |
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| 作者单位： | Chemistry Department of Life and Environmental Science College, Shanghai Normal University, Shanghai 200234 |
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| 基金项目： | 上海市自然科学基金（002A14042），上海市科学技术委员会资助项目（011511021） |
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| 摘 要： | 利用原位电化学及表面增强拉曼散射（SERS）光谱方法对寡聚脱氧核苷酸（26-mers ODN和13-mers ODN）在银电极表面上的吸附状态进行了研究. 实验表明, 单链寡聚脱氧核苷酸在银电极上有很好的SERS光谱,单链寡聚脱氧核苷酸在银表面上主要以碱基腺嘌呤（A）为吸附点,吸附状态随电位变化而变化, 链长较短的寡聚脱氧核苷酸在银电极表面的吸附态对电位变化较敏感.
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| 关 键 词： | 寡聚脱氧核苷酸(ODN) 表面增强拉曼光谱 腺嘌呤 吸附 银电极 |
| 收稿时间： | 2001-10-16 |
| 修稿时间： | 2001-10-16 |
Change of Adsorption State of Oligodeoxynucleotides on the Silver Electrode Surface with Change of Potential |
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| Shen He-Bai Zhou Wen-Jun Yang Hai-Feng Yu Pei-Tao. Change of Adsorption State of Oligodeoxynucleotides on the Silver Electrode Surface with Change of Potential[J]. Acta Physico-Chimica Sinica, 2002, 18(6): 490-494. DOI: 10.3866/PKU.WHXB20020603 |
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| Authors: | Shen He-Bai Zhou Wen-Jun Yang Hai-Feng Yu Pei-Tao |
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| Affiliation: | Chemistry Department of Life and Environmental Science College, Shanghai Normal University, Shanghai 200234 |
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| Abstract: | The oligodeoxynucleotides is anchoraged on the silver electrode surface by means of physical methods. The in-situ Electrochemistry-Surface Enhanced Raman Scattering (SERS) Spectrum is used to study the adsorption state of 26-mers oligodeoxynucleotides (ODN) and 13-mers ODN on the electrode surface respectively. The SERS spectra of oligdeoxynucleotides based on the silver electrode have obtained. The results of study of the SERS spectra change of ODN with the change of potential on the silver electrode demonstrate that the oligodeoxynucleotides adsorbed on the silver electrode surface primarily through the special base-Adenine (A). The adsorption state changes with the variation of the potential on the electrode. The shorter ODN changes more quickly and is more sensitive to the change of potential than the longer ODN(see Table 1). |
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| Keywords: | Oligodeoxynucleotide (ODN) SERS Adenine Adsorption Silver electrodes |
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