Separation by capillary zone electrophoresis of the active anthraquinone components of the Chinese herbPolygonum multiflorum Thunb |
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Authors: | Dexian Wang Gengliang Yang H Engelhardt Haixing Liu Jingxiang Zhao |
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Institution: | (1) Departament de Química Analitica, Facultat de Química, Universitat de València, Doctor Moliner 50, 46100 Burjassot, València, Spain;(2) Departamento de Química y Edafología, Facultad de Ciencias, Universidad de Navarra, Irunlarrea s/n, 31080 Pamplona, Spain |
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Abstract: | Summary An optimization strategy was applied to explore the capability of hybrid micellar eluents of sodium dodecyl sulphate (SDS),
using acetonitrile or pentanol as modifiers, to resolve mixtures of eleven steroids showing a wide range of hydrophobicity
(clostebol acetate, dehydrotestosterone, dydrogesterone, medroxyprogesterone, medroxyprogesterone acetate, methandienone,
methyltestosterone, progesterone, testosterone, testosterone enanthate and testosterone propionate). The accurate prediction
of the retention behaviour of the steroids, with relative errors in the 0.8–1.7% and 0.4–2.9% ranges for SDS-acetonitrile
and SDS-pentanol eluents, respectively, demonstrated the reliability of the methodology. Acetonitrile and pentanol had a complementary
effect in these analyses. The elution strength of acetonitrile was weaker, but allowed higher efficiencies. A 0.094 M SDS-16%
acetonitrile eluent separated seven steroids (dehydrotestosterone, dydrogesterone, medroxyprogesterone, medroxyprogesterone
acetate, methandienone, methyltestosterone and testosterone) in 27 min, while the most hydrophobic steroids were strongly
retained. In contrast, a 0.125 M SDS-5.8% pentanol eluent permitted the elution of a mixture of eight steroids (dehydrotestosterone,
dydrogesterone, medroxyprogesterone acetate, methandienone, methyltestosterone, progesterone, testosterone enanthate and testosterone
propionate) of diverse hydrophobicity in 14 min. With this eluent, however, the peaks of dehydrotestosterone-medroxyprogesterone
and methandienone-testosterone were highly overlapping. |
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Keywords: | Column liquid chromatography Micellar eluents Steroidal hormones Sodium dodecyl sulphate |
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