Optimization of the chromatographic determination of polyphenols in the aerial parts ofCichorium intybus L. |
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| Authors: | N Mulinacci M Innocenti S Gallori A Romani G la Marca F F Vincieri |
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| Institution: | (1) Dipartimento di Scienze Farmaceutiche, Università di Firenze, Via G. Capponi 9, 50121 Florence, Italy |
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| Abstract: | Summary The aim of this work was to contribute to the phytochemical characterization ofCichorium intybus L. var.silvestre, chicory. Semi-preparative HPLC analysis was applied to an extract of fresh wild chicory leaves to separate and collect the
main polyphenolic compounds.
HPLC-diode-array detection (DAD), HPLC-MS, and NMR were used for the complete chemical characterization of all the compounds
isolated. The molecules characterized were monocaffeoyl tartaric acid, chicoric acid, monocaffeolyp-hydroxycinnamoyl tartaric acid, caffeoyl feruloyl tartaric acid, chlorogenic acid, quercetin-3-O-glucuronide, luteolin-7-O-glucuronide, and quercetin-3-O-glucoside.
The chromatographic behaviour of the main components of the extract of the leaves has been compared on three different stationary
phases-LiChrosorb RP18, Luna C18, and Luna Phenyl-Hexyl. |
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| Keywords: | Column liquid chromatography-DAD and column liquid chromatography-MS Flavonoids Polyphenols Chicoric acid Cichorium intybus L |
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