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Influence of Heat Treatment on Biological Properties of Chitosan toward Vascular Cells in vitro |
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| Authors: | Sae-Hwan Lim Ki-Sook Park Hyun-Sook Park Yong-Jae Gin In-Sup Noh Chan-Woong Park Young-Sook Son Chun-Ho Kim |
| Institution: | 1. Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, Seoul 139-240, Korea, Fax: (+82)-2-977-0381
R & D Institute, Modern Tissue Technologies Inc., Seoul 139-706, Korea;2. R & D Institute, Modern Tissue Technologies Inc., Seoul 139-706, Korea;3. Laboratory of Tissue Engineering, Korea Institute of Radiological and Medical Sciences, Seoul 139-240, Korea, Fax: (+82)-2-977-0381;4. Chemical Engineering Department, Seoul National University of Technology, Seoul 151-741, Korea |
| Abstract: | The effects of heat treatment of chitosan film on the proliferation of vascular cells were examined in vitro. Chitosan film was heat treated at 110 °C in vacuo for 1∼3 days. Heat treatment slightly improved the hydrophilicity of chitosan, although the cross-linking of chitosan took place after heat treatment. Chitosan films were cytocompatible toward smooth muscle cells (SMCs) and human dermal microvascular endothelial cells (HDMECs) before and after heat treatment of chitosan. In particular, the heat treated chitosan supported the proliferation of both vascular cell types for one day. The growth of HDMECs was greater than that of SMCs on heat treated chitosan film for one day. Heat treated chitosan has intrinsic biological activity which could contribute to a retardation of SMC growth relative to HDMECs. |
| Keywords: | biocompatibility chitosan endothelial cells heat treatment smooth muscle cells |