Characterization of a Hg2+-Selective Fluorescent Probe Based on Rhodamine B and Its Imaging in Living Cells |
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Authors: | Wenting Zhang Chunwei Yu Mei Yang Shaobai Wen Jun Zhang |
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Affiliation: | 1.Laboratory of Environmental Monitoring, School of Tropical and Laboratory Medicine, Hainan Medical University, Haikou 571199, China; (W.Z.); (C.Y.); (S.W.);2.School of Public Health, Hainan Medical University, Haikou 571101, China;3.Laboratory of Tropical Biomedicine and Biotechnology, Hainan Medical University, Haikou 571101, China |
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Abstract: | A small organic molecule P was synthesized and characterized as a fluorometric and colorimetric dual-modal probe for Hg2+. The sensing characteristics of the proposed probe for Hg2+ were studied in detail. A fluorescent enhancing property at 583 nm (>30 fold) accompanied with a visible colorimetric change, from colorless to pink, was observed with the addition of Hg2+ to P in an ethanol-water solution (8:2, v/v, 20 mM HEPES, pH 7.0), which would be helpful to fabricate Hg2+-selective probes with “naked-eye” and fluorescent detection. Meanwhile, cellular experimental results demonstrated its low cytotoxicity and good biocompatibility, and the application of P for imaging of Hg2+ in living cells was satisfactory. |
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Keywords: | Hg2+ fluorescent probe rhodamine B cell imaging |
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