A Non-Invasive Hair Test to Determine Vitamin D3 Levels |
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| Authors: | Iltaf Shah Mohammad Mansour Sheikh Jobe Emadaldeen Salih Declan Naughton Syed Salman Ashraf |
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| Affiliation: | 1.Department of Chemistry, College of Science, UAE University, Al Ain P.O. Box 15551, United Arab Emirates; (M.M.); (S.J.); (E.S.);2.School of Life Sciences, Pharmacy and Chemistry, Kingston University London, Surrey KT1 2EE, UK;3.Department of Chemistry, College of Arts and Sciences, Khalifa University, Abu Dhabi P.O. Box 127788, United Arab Emirates |
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| Abstract: | Vitamin D deficiency is being recognized as a global issue and has been implicated in many health issues. Hence, there is an increased interest in developing sensitive, reproducible, and non-invasive assays to measure Vitamin D levels. This study aimed to apply a sensitive liquid chromatography-mass spectrometric assay to hair samples to develop and validate a clinical assay to provide a quarterly average level of vitamin D in one test. Hair samples were collected from 70 male university students/young adults and pulverized/sonicated in methanol/water for 2 h to extract Vitamin D metabolites. A sensitive liquid chromatographic-mass spectrometric assay was employed to quantitate vitamin D and metabolites. Of the eight Vitamin D and metabolites screened, only the primary, clinically significant form of vitamin D (25OHD3) was detected and quantified in hair samples in the range of 17–1541 pg/mg. One-third of the hair samples (21 out of 70) had Vitamin D levels below the LLOD of the assay (10 pg/mg). The mean and standard deviation values for hair (25OHD3) were 276.7 ± 329.9, respectively. This pilot study reveals the potential of the vitamin D hair test in clinical assays as a complementary test to a vitamin D blood test, which would provide a quarterly average. |
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| Keywords: | vitamin D assay hair analyses liquid chromatography-mass spectrometry |
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