| 固定化有机改进剂RP－HPLC固定相的研究 |
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| 引用本文： | 左雄军, 徐友志, 徐秉坤, 汤澍. 固定化有机改进剂RP－HPLC固定相的研究[J]. 分析测试技术与仪器, 1995, (4): 32-35. |
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| 作者姓名： | 左雄军 徐友志 徐秉坤 汤澍 |
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| 作者单位： | 1.湖南师范大学分析测试中心 |
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| 摘 要： | 通过在RPHPLC固定相C18表面键合氨基硅烷,引入氨基基团,将有机改进剂固定至硅胶键合相表面,克服了固定相表面硅羟基对含氮有机化合物的不可逆吸附.以最简单的CH3OH-H2O洗脱体系即能获得含氮有机物的最佳分离,从而可简化分离操作,提高柱寿命,降低分离成本.
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| 关 键 词： | 硅胶基18烷酰胺填料 RP－HPLC 柱评价 固定化 有机改进剂 |
| 收稿时间： | 1995-07-26 |
| 修稿时间： | 1995-09-11 |
Preparation of RP-HPLC Packing with Immobilized Organic Modifier |
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| Zuo Xiongjun, Xu Youzhi, Xu Bingkun, Tang Shu. Preparation of RP-HPLC Packing with Immobilized Organic Modifier[J]. Analysis and Testing Technology and Instruments, 1995, (4): 32-35. |
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| Authors: | Zuo Xiongjun Xu Youzhi Xu Bingkun Tang Shu |
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| Affiliation: | 1.Analysis and Test Center of Hunan Nounal University, Changsha 410081 |
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| Abstract: | By the reaction of aminopropyl triethoxy silane bonded silica phase with octodecanoyl chloride, a RP-HPLC packing with immobilized organic modifier for the separation of nitrogen-containing organic compounds was obtained. With this packing the separation of neutral organics is as good as the thaditional C18 bonded phase while for good separation of basic organics neither inorganic salts nor organic modifiers such as organic acids or bases are required in the mobile phase. |
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| Keywords: | Silica-based stearamide packing RP-HPLC Column evaluation Immobilization Organic modifier. |
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