Determination of selenium status using the nail biologic monitor in a canine model |
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Authors: | J Steven Morris Vickie L Spate Ruth Ann Ngwenyama David J Waters |
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Institution: | (1) Research Reactor Center, University of Missouri, 1513 Research Park Drive, Columbia, MO 65211, USA;(2) Harry S. Truman Memorial Veterans Hospital, Columbia, MO 65211, USA;(3) Comparative Oncology Program, Department of Veterinary Clinical Sciences, Purdue University, West Lafayette, IN, USA;(4) Gerald P. Murphy Cancer Foundation, West Lafayette, IN 47906, USA;(5) Center on Aging and the Life Course, Purdue University, Ernest C. Young Hall, 155 South Grant Street, West Lafayette, IN 47907-2114, USA |
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Abstract: | Toenails and fingernails are routinely used to estimate selenium status in epidemiological studies; however, literature validating
nail selenium concentration as a surrogate for critical organs is limited. In this study diets of intact male dogs were selenium
supplemented at two physiological levels (3 and 6 μg/kg/day) in two different forms, selenomethionine and selenium-enriched
bioformed yeast. The selenium-adequate basal diet consumed by the treatment and control groups during the 4-week run-in period
and throughout the trial contained 0.3 ppm selenium. After 7 months the dogs in the two treatment groups and the control group
were euthanized. Representative tissue samples from prostate, brain, liver, heart and skeletal muscle were collected, rinsed
and frozen. Toenail clippings from multiple toes were also collected. Selenium was determined by neutron activation analysis
using Se77m (half life = 17.4 s) at the University of Missouri Research Reactor Center. NIST SRM 1577, Bovine Liver was analyzed
as a quality control. The analysts were blinded to control and treatment group assignments. As expected, tissue selenium levels
increased proportionally with supplementation. A slightly greater increase in tissue selenium was observed for the purified
selenomethionine compared to the bioformed yeast; however this trend was significant only for brain tissue. Toenail selenium
concentrations and tissue selenium were highly correlated (p < 0.003) with Pearson coefficients of 0.759 (skeletal muscle), 0.745 (heart), 0.729 (brain), 0.723 (prostate), and 0.632
(liver). The toenail biologic monitor accurately assesses selenium status in skeletal muscle, heart, brain, prostate, and
liver in the canine model. |
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