A method for the making and utility of gadolinium-labeled albumin microbubbles |
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Authors: | Anderson Daniel R Duryee Michael J Garvin Robert P Boska Michael D Thiele Geoffrey M Klassen Lynell W |
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Institution: | a Experimental Immunology, Research in Cardiovascular Disease Laboratory at the University of Nebraska Medical Center, Division of Cardiology, Department of Internal Medicine, 982265 Nebraska Medical Center, Omaha, NE 68198-2265, USAb Experimental Immunology, Research in Autoimmune Disease Laboratory at the Omaha VA Medical Center, Research Services 151, Omaha, NE 68124, USAc Experimental Immunology, Research in Rheumatologic Disease Laboratory at the University of Nebraska Medical Center, Division of Rheumatology, Department of Internal Medicine, 983025 Nebraska Medical Center, Omaha, NE 68198-3025, USAd Bioimaging Core Laboratory at the University of Nebraska Medical Center, Department of Radiology, 981045 Nebraska Medical Center, Omaha, NE 68198-1045, USAe University of Nebraska Medical Center, Department of Pathology and Microbiology, 986495 Nebraska Medical Center, Omaha, NE 68198-6495, USA |
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Abstract: | ObjectivePerfluorocarbon-exposed sonicated dextrose albumin microbubbles (PESDA) binds scavenger receptors and can be noninvasively imaged. To enhance imaging, gadolinium (Gd)-labeled PESDA was developed and tested in a model of vascular inflammation by magnetic resonance imaging (MRI).Methods and ResultsPurified human serum albumin (HSA) (5%) was labeled with Gd via the covalent binding of diethylenetriaminepentacetic acid. Abdominal aortic tissues in Sprague-Dawley rats (n=5 per group) were analyzed by 7-T MRI and scanning electron microscopy to evaluate PESDA binding. Labeling-purified 5% human albumin resulted in an average of 16.1 Gd atoms per albumin molecule as determined by atomic absorption. Forty-eight hours after balloon angioplasty, aortic tissue was enhanced with Gd-PESDA as compared to control tissue. 7-T MRI of explanted tissues was sensitive to the detection of retained PESDA. Enhancement of aortic tissue in vivo was present albeit to a lesser extent than explanted tissue from the same animals.ConclusionsHSA was successfully labeled, and an albumin-based microbubble with Gd was synthesized. This contrast agent, Gd-PESDA, may serve as an additional agent for the MRI evaluation of innate inflammation and used to noninvasively image early vascular pathophysiologic processes.Condensed AbstractIn this study, Gd-PESDA microbubbles and were synthesized and shown to detect the binding of these microbubbles using MRI in injured aortic tissue. The method for synthesizing Gd-PESDA is detailed, and the proposed utility of this new contrast agent is discussed. |
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Keywords: | Gadolinium Magnetic Resonance Imaging MRI Perfluorocarbon exposed sonicated dextrose albumin PESDA Vascular inflammation Scavenger receptors Atherosclerosis |
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