Analysis of the characteristic PSP profiles ofPyrodinium bahamense and several strains ofAlexandrium by HPLC based on ion-pair chromatographic separation, post-column oxidation, and fluorescence detection |
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Authors: | C. Hummert M. Ritscher R. Reinhardt B. Luckas |
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Affiliation: | (1) Institute of Nutrition and Environment, University of Jena, Dornburger Str. 25a, 07743 Jena, Germany |
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Abstract: | Summary A sensitive HPLC method for determination of paralytic shellfish poisoning (PSP) based on ion-pair chromatographic separation of PSP toxins, post-column oxidation with periodic acid, and fluorescence detection has been used to determine toxin profiles ofPyrodinium bahamense and several strains ofAlexandrium. The HPLC chromatograms revealed clear differences betweenPyrodinium bahamense andAlexandrium strains. Presented at the 21st ISC held in Stuttgart, Germany, 15th–20th September, 1996. |
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Keywords: | Column liquid chromatography (CLC) Ion-pair chromatography Post-column oxidation Paralytic shellfish poisons (PSP) Alexandrium Pyrodinium |
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