Hyphenated techniques in anticancer drug monitoring. I. Capillary gas chromatography-mass spectrometry |
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Authors: | Guetens G De Boeck G Wood M Maes R A A Eggermont A A M Highley M S van Oosterom A T de Bruijn E A Tjaden U R |
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Affiliation: | Department of Microbiology and Immunology, University of South Carolina, School of Medicine, Columbia 29208, USA. |
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Abstract: | Muramic acid (Mur) is present in the cell wall of Eubacteria and serves as a chemical marker for the trace detection of bacteria and bacterial cell wall debris in complex matrices. There have been numerous studies using a variety of derivatives of Mur, particularly in combination with gas chromatography-tandem mass spectrometry (GC-MS-MS) where the detection limit has been steadily lowered. A stable, halogenated derivative, the pentafluorobenzyl oxime (PFBO) acetate of Mur, has been developed by others and successfully used for GC with electron-capture detection. The current report is the first use of this derivative for GC-MS-MS analysis of Mur, or indeed any other carbohydrate, using negative ion chemical ionization (NICI) with GC-MS-MS. Mur was readily detected in settled surface dust (166 ng/mg), as well as dust collected from indoor air (1.4-5.9 ng/mg). Analyses of Mur as a PFBO acetate by GC-NICI-MS-MS or as alditol acetates by electron impact GC-electron impact ionization MS-MS serve as complementary approaches for trace detection in complex matrices. |
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