大鼠端脑神经元型一氧化氮合酶免疫阳性神经元的分布

目的 :观察大鼠端脑神经元型一氧化氮合酶 (nNOS)免疫阳性神经元的分布。方法 :ABC免疫细胞化学法显示大鼠端脑nNOS阳性神经元。结果 :nNOS阳性神经元呈棕褐色 ,胞体的形态多样化 ,呈梭形、三角形、圆形等多种形状 ,突起有一个或多个 ;阳性纤维呈棕色串珠状 ,个别脑区阳性纤维相互交织成网。端脑nNOS免疫阳性神经元主要分布于嗅结节 (包括海马回 )、梨状区 (包括梨状皮质和梨状核 )、斜角带、隔区、杏仁复合体、海马结构、尾壳核和苍白球 ,以及大脑皮质等各个部位 ,其中以大脑皮质、梨状区和斜角带、尾壳核等部位最为丰富。结论 :大鼠端脑内分布有丰富的nNOS阳性神经元 ,它们可能通过调节神经递质的分泌 ,参与脑的高级整合功能。

Distribution of neuronal nitric oxide synthase immunopositive neurons in cerebrum of rats

Aim: To observe the distribution of neuronal nitric oxide synthase (nNOS) immunopositive neurons in cerebrum of rats. Methods: Using ABC immunocytochemistry technique displayed the nNOS immunopositive neurons. Results: nNOS immunopositive neurons were brown and had one to four processes, their shapes were diversiform such as fusiform, triangle spherical. The immunopositive fibers were strings of pearls, and crossed each other. The nNOS immunopositive neurons in cerebrum of rats were mainly located in olfactory tubercle (including Calleja island), piriform area (including pyriform cortex and pyriform nucleus), diagonal band, septal area, almond complex, hippocampal formation, caudate putamen, globus pallidus, and cerebral cortex. Especially, the nNOS immunopositive neurons were more abundant in cerebral cortex, Piriform area, diagonal band, and cautate putamen. Conclusion: There were many nNOS immunopositive neurons in cerebrum of rats, and they might take part in the high-level integration of brain by regulating secretion of neurotransmitters.