Bioprocess Optimization of Furanocoumarin Elicitation by Medium Renewal and Re-elicitation: A Perfusion-Based Approach |
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Authors: | Renuka Diwan Nutan Malpathak |
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Institution: | (1) Department of Botany, University of Pune, Pune, 411007, Maharashtra, India; |
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Abstract: | Effect of various abiotic (methyl jasmonate, salicylic acid) and biotic (yeast extract, Aspergillus niger) elicitors on furanocoumarin production and in situ product removal was studied using shoot cultures of Ruta graveolens L. Elicitation by yeast extract (1% w/v) on day 15 was most effective. It led to 7.8-fold higher furanocoumarin production that was attained 24 h after elicitation
and 43% of the product was released into the medium. Changes in the relative concentration of furanocoumarins produced depend
on the elicitor used. Molar ratio of bergapten increased to 93% in response to yeast extract. With the perspective of developing
a commercially feasible process, an approach for preserving viability of biomass and its reuse needs to be developed. For
this, medium renewal strategy was investigated. Removal of the spent medium 48 h after elicitation allowed in situ product
removal and proved effective in revival of cultures, allowing reuse of biomass. A week after medium renewal, the revived biomass
was re-elicited and a second furanocoumarin production peak was obtained. A perfusion-based bioprocess optimization approach,
employing elicitation coupled with medium renewal with subsequent re-elicitation, as a new strategy for improved furanocoumarin
production, has been suggested. |
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