| Assay of Cysteine in Human Serum with Quinine-Ce~(4+) Chemiluminescence System |
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| 作者姓名： | Li Hua NIE Hui Min MA* Mei Hong SU Ming SUN Xiao Hua LI Center for Molecular Sciences Institute of Chemistry Chinese Academy of Sciences Beijing |
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| 作者单位： | Li Hua NIE，Hui Min MA*，Mei Hong SU，Ming SUN，Xiao Hua LI Center for Molecular Sciences，Institute of Chemistry，Chinese Academy of Sciences，Beijing 100080 |
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| 基金项目： | 国家自然科学基金，中国科学院科研项目 |
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| 摘 要： | Cysteine is one of amino acids containing thiol groups. It not only constitutes protein but also plays an important role in several biological processes, such as redox, methyl transfer and carbon fixation reactions in which CoA participates. Therefore, the detection of cysteine and its derivatives has attracted much attention1-3. In this work, a CL method was developed for the determination of cysteine, which was based on oxidation of the thiol group of cysteine by cerium sulfate. This r…
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Assay of Cysteine in Human Serum with Quinine-Ce4+ Chemiluminescence System |
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| Li Hua NIE,Hui Min MA*,Mei Hong SU,Ming SUN,Xiao Hua LI Center for Molecular Sciences,Institute of Chemistry,Chinese Academy of Sciences,Beijing .Assay of Cysteine in Human Serum with Quinine-Ce~(4+) Chemiluminescence System[J].Chinese Chemical Letters,2002,13(10). |
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| Authors: | Li Hua NIE Hui Min MA Mei Hong SU Ming SUN Xiao Hua LI |
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| Institution: | Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080 |
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| Abstract: | A sensitive and selective chemiluminescence (CL) method was developed for the determination of cysteine. This method is based on that the weak CL of cysteine oxidized with cerium (IV) can be greatly enhanced by quinine, and the total cysteine in human serum can be detected through simply diluting with water, showing a simpler analytical characteristic. |
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| Keywords: | Chemiluminescence cysteine quinine-Ce4 system human serum |
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