毛细管电泳应用于测定牛血清白蛋白与脂质体的相互作用

建立了一种用毛细管电泳法检测牛血清白蛋白(BSA)与脂质体相互作用的分析方法。氧化指数的测定实验结果表明经过冷冻干燥的脂质体稳定性更好;毛细管电泳表征脂质体的电荷性质实验结果表明脂质体在pH 5.0～8.0的条件下呈电中性。在pH 7.0的条件下,以各种浓度的脂质体混悬液为电泳缓冲液,以0.8%二甲亚砜(DMSO)为内标,随着缓冲液中脂质体质量浓度从0增加到2.4 mg/mL,BSA的有效淌度从-2.232×10-4 cm2·V－1·s－1变化到-3.046×10-4 cm2·V－1·s－1;结合Scatchard分析,测得BSA与脂质体的结合常数为2.522×103(g/mL)－1。该方法简单、快速,为研究蛋白质与脂质体的相互作用提供了新的技术手段。

Investigation on interaction between bovine serum albumin and liposome using capillary electrophoresis

A method for the investigation on the interaction between bovine serum albumin (BSA) and liposome using capillary electrophoresis was developed. The oxidation index showed that liposomes undergoing freeze-drying were more stable. The results obtained from the capillary electrophoretic analysis of liposome showed that liposome had no charge at pH 5.0-8.0. A series of liposome suspension at different concentrations with the internal marker of 0.8% dimethyl sulfoxide (DMSO) were introduced as the electrophoresis buffer at pH 7.0. Along with the liposome concentrations increased from 0 to 2.4 mg/mL, it was found that the effective mobility of BSA reduced from −2.232 × 10⁻⁴ cm²·V⁻¹·s⁻¹ to −3.046 × 10⁻⁴ cm²·V⁻¹·s⁻¹. The binding constant between BSA and liposome was 2.522 × 10³ (g/mL)⁻¹, which was calculated using Scatchard analysis. This method is simple and rapid and provides a new technology for the investigation on the interactions between protein and liposome.