Aminopropargyl derivative of terpyridine-bis(methyl-enamine) tetraacetic acid chelate of europium (Eu (TMT)-AP3): a new reagent for fluorescent labelling of proteins and peptides |
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Authors: | Poupart Séverine Boudou Cédric Peixoto Philippe Massonneau Marc Renard Pierre-Yves Romieu Anthony |
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Institution: | IRCOF/LHO, Equipe de Chimie Bio-Organique, UMR 6014 CNRS, INSA de Rouen et Université de Rouen, 1, rue Lucien Tesnijres, FR-76131 Mont-Saint-Aignan Cedex, France. |
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Abstract: | The synthesis and photophysical properties of a new terpyridine-based europium(III) chelate (Eu (TMT)-AP3) designed for peptide and protein labelling in aqueous solution phase is described. In order to obtain a stable, easy to handle, versatile and efficient labelling agent, a reactive aminopropargyl arm has been introduced onto the terpyridine moiety. As preliminary biochemical applications the chelate has been 1) efficiently covalently attached onto a representative biomolecule-monoclonal antibody-and 2) converted into iodoacetamido and aldehyde derivatives, and the photoluminescent Eu (TMT)-AP3 was grafted onto cysteine and lysine amino acid residues respectively. These two different solution phase labelling methods yielded original fluorogenic FRET based probes suitable for "in vitro" detection of caspase-3 protease, a key mediator of apoptosis of mammalian cells. |
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