Thin chip microsprayer system coupled to quadrupole time-of-flight mass spectrometer for glycoconjugate analysis |
| |
Authors: | Zamfir Alina D Lion Niels Vukelic Zeljka Bindila Laura Rossier Joel Girault Hubert H Peter-Katalinic Jasna |
| |
Institution: | Institute for Medical Physics and Biophysics, Biomedical Analysis, University of Munster, Germany. zamfir@uni-muenster.de |
| |
Abstract: | A thin chip polymer-based microsprayer has been coupled to a hybrid quadrupole time-of-flight mass spectrometer (QTOF MS) and introduced in carbohydrate research. The feasibility of the approach is demonstrated for mapping, sequencing and structural elucidation of glycoconjugates originating from human body fluids and tissues such as a glycopeptide mixture from normal human urine and an isolated and purified GT1 ganglioside fraction from normal adult human brain. The optimization procedure required by each glycoconjugate category is described and the advantages of the system in terms of flexibility and adaptability to QTOF MS, stability of the ESI MS signal, carbohydrate ionization and sequencing, sensitivity, speed of analysis and sample consumption are discussed. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|