| 血红蛋白在银溶胶上的表面增强喇曼光谱研究 |
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| 引用本文： | 冶保献,林琳,张悟铭,周性尧. 血红蛋白在银溶胶上的表面增强喇曼光谱研究[J]. 高等学校化学学报, 1998, 19(8): 1239-1241 |
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| 作者姓名： | 冶保献 林琳 张悟铭 周性尧 |
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| 作者单位： | 1. 郑州大学化学化工学院, 郑州, 450052;2. 武汉大学化学系, 武汉, 430072 |
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| 摘 要： | 表面增强喇曼光谱已广泛应用于物质分子在金属表面吸附的研究.人们发现,不仅无机物和有机小分子能产生SERS,而且生物分子,如核酸、色蛋白以及蛋白质均能产生SERS效应,并以此来研究生物分子-蛋白质的变性问题.
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| 关 键 词： | 血红蛋白 血红素 表面增强喇曼光谱 |
| 收稿时间： | 1997-10-16 |
A Study of Hemoglobin at Silver Colloid by Surface Enhanced Raman Spectroscopy |
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| YE Bao-Xian,LIN Lin,ZHANG Wu-Ming,ZHOU Xing-Yao. A Study of Hemoglobin at Silver Colloid by Surface Enhanced Raman Spectroscopy[J]. Chemical Research In Chinese Universities, 1998, 19(8): 1239-1241 |
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| Authors: | YE Bao-Xian LIN Lin ZHANG Wu-Ming ZHOU Xing-Yao |
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| Affiliation: | 1. College of Chemistry and Chemical Engineering, Zhengzhou University, Zhengzhou, 450052;2. Department of Chemistry, Wuhan University, Wuhan, 430072 |
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| Abstract: | The properties of Hemoglobin adsorbed on the silver colloid surface were investigated by using surface enhanced Raman spectroscopy(SERS) in the determination of oxygen hemoglobin, deoxygen hemoglobin and heme. The results show that the heme chromophore of oxygen hemoglobin interacts directly with silver colloid, as a result of either its detachment from the protein matrix or denaturation from the protein. The deoxygen hemoglobin would not release its heme at the silver colloid as normally expected. |
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| Keywords: | Hemoglobin Heme Surface enhanced Raman spectroscopy |
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