HPLC柱切换技术用于同时分析鱼腥草中3种黄酮类成分

利用柱切换技术解决了鱼腥草中难分离组分的分离和低含量组分的定量问题。以Synergi Fusion-RP柱(250 mm×4.6 mm i.d.,4μm)作为预柱,甲醇-0.05%磷酸溶液作流动相,以Acclaim120 C18柱(250mm×4.6 mm i.d.,5μm)作为分析柱,乙腈-0.05%磷酸溶液作流动相,对鱼腥草中的3种黄酮类成分进行分析。该方法在10.0～1 000.0 mg.L-1范围内线性关系良好,相关系数(r)不低于0.998,回收率为95%～99%,RSD值均小于3%。该方法操作简便、快速,可作为分析鱼腥草中黄酮类组分的有效手段。

Simultaneous Separation and Analysis of 3 Flavonoid Components in Houttuynia cordata Thunb by Column Switching Technology

A method was established for the separation of complex and low content components in Houttuynia cordata Thunb by column-switching technology employing two reverse phase liquid chromatographic columns.A Synergi Fusion-RP column and an Acclaim120 C18 column with 250 mm×4.6 mm i.d.were adopted as the precolumn and analytical column,respectively.The precolumn was eluted with methanol-0.05% phosphoric acid as mobile phase.The analytical column was eluted with 0.05% phosphoric acid-acetonitrile.Three flavonoid components in Houttuynia cordata Thunb were simultaneously separated and detected.The calibration curves were linear in the concentration range of 10.0-1 000.0 mg·L-1,with correlation coefficient not less than 0.998.The recoveries of three compounds in Houttuynia cordata Thunb ranged from 95% to 99% with RSDs less than 3%.The results showed that the developed method was simple and rapid,and could be used in the analysis of flavonoid components.