聚对氨基苯磺酸修饰电极对桑枝中桑色素的灵敏测定

采用电聚合的方法制备了聚对氨基苯磺酸(PABSA)修饰电极，以循环伏安法和差分脉冲伏安法研究了桑色素在该修饰电极上的电化学行为。PABSA和黄酮类药物桑色素的π-π共轭作用使得桑色素在该修饰电极上产生的氧化峰更加灵敏。实验发现，在pH 7.0的磷酸盐缓冲介质中，桑色素在0.214 V处产生灵敏的氧化峰。在优化实验条件下，采用差分脉冲伏安法对桑色素进行定量测定，桑色素的氧化峰电流与其浓度呈良好的线性关系，线性范围为5.0×10-7~1.0×10-3 mol/L，检出限为1.0×10-7 mol/L。将该修饰电极用于桑枝生物样品中桑色素含量的测定，结果满意。该方法具有灵敏度高、重现性好的特点，且该修饰电极稳定性高，可重复使用。

Sensitive Determination of Morin in Ramulus Mori on the Poly(p-aminobenzene sulfonic acid) Modified Glassy Carbon Electrode

A novel PABSA modified glassy carbon electrode(PABSA/GCE) was fabricated by electropolymerizing PABSA on the surface of GC electrode.Morin can effectively accumulate on the electrode for the π-π stacking reaction between the aromatic rings of morin and the dimers of PABSA and cause an increased anodic peak signal.Based on this,a method was developed for the determination of morin.The electrochemical behaviors of morin on the modified electrode were investigated using cyclic voltammetry(CV) and differential pulse voltammetry(DPV).The results showed that,in pH 7.0 PBS,morin generated a sensitive anodic peak at 0.214 V.Under the optimized conditions,DPV was used for detecting morin.The calibration curve was linear in the range of 5.0×10-7-1.0×10-3 mol/L,with a detection limit of 1.0×10-7 mol/L.The modified electrode,with the advantages of good stability,repeatability and wide linear range,was successfully applied in the determination of morin content in ramulus mori.