Method for the characterization of stationary phases for the separation of proteins by high-performance liquid chromatography |
| |
| Authors: | R E Huisden J C Kraak H Poppe |
| |
| Affiliation: | Laboratory for Analytical Chemistry, University of Amsterdam, The Netherlands. |
| |
| Abstract: | A conformational change of lysozyme and myoglobin can be visualized with transition curves in a high-performance liquid chromatographic (HPLC) system using a diode-array detector and UV difference spectrometry. Transition temperatures calculated from the curves are affected by the mobile phase composition and the column packing material. Flow-rates as used in HPLC do not have a measurable influence. |
| |
| Keywords: | |
|
