Process development for production of medium chain triglycerides using immobilized lipase in a solvent-free system |
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Authors: | Langone Marta A P Sant’Anna Geraldo L |
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Institution: | 1.Instituto de Química da Universidade do Estado do Rio de Janeiro, Rua Sa? Francisco Xavier 524, PHLC, SL 310, CEP 20550-013, Rio de Janeiro, RJ, Brazil ;2.COPPE, Universidade Federal do Rio de Janeiro, PO Box 68502, CEP 21945-970, Rio de Janeiro, RJ, Brazil ; |
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Abstract: | The synthesis of tricaprylin, tricaprin, trilaurin, and trimyristin in a solvent-free system was conducted by mixing a commercial
immobilized lipase with the organic reagents (glycerol and fatty acid) in a 20-mL batch reactor with constant stirring. The
effects of temperature, fatty acid/glycerol molar ratio, and enzyme concentration on the reaction conversion were determined.
The reactions were carried out for 26 h and the nonpolar phase was analyzed by gas chromatography. Appreciable levels of medium
chain triglycerides were achieved, except for tricaprylin. The higher selectivity values for the production of triglycerides
were attained under the following conditions: a fatty acid/glycerol molar ratio of 5; enzyme concentration of 5 or 9% (w/w);
and temperatures of 70°C (tricaprin), 80°C (trilaurin), and 90°C (trimyristin). After completion of the esterification reaction
under these conditions, the recovery of the triglyceride and fatty acids, and the reusability of the enzyme were studied.
The unreacted fatty acid and the produced triglyceride were satisfactorily recovered. The commercial immobilized lipase was
used in 10 consecutive batch reactions at 80°C, with 100% selectivity in the trilaurin and trimyristin synthesis. The possibility
of enzyme reuse and the recovery of residual fatty acid are relevant results that contribute to increasing the viability of
the process. |
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Keywords: | Medium chain triglycerides immobilized lipase esterification |
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