Optimization and single-laboratory validation study of a high-performance liquid chromatography (HPLC) method for the determination of phenolic Echinacea constituents |
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Authors: | Paula N Brown Michael Chan Joseph M Betz |
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Institution: | (1) British Columbia Institute of Technology, 3700 Willingdon Avenue, Burnaby, BC, V5G-3H2, Canada;(2) Office of Dietary Supplements, National Institutes of Health, Bethesda, MD 20892, USA |
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Abstract: | Three species of Echinacea (Echinacea purpurea, Echinacea angustifolia, and Echinacea pallida) are commonly used for medicinal purposes. The phenolic compounds caftaric acid, cichoric acid, echinacoside, cynarin, and
chlorogenic acid are among the phytochemical constituents that may be responsible for the purported beneficial effects of
the herb. Although methods for the analysis for these compounds have been published, documentation of their validity was inadequate
as the accuracy and precision for the detection and quantification of these phenolics was not systematically determined and/or
reported. To address this issue, the high-performance liquid chromatography method, originally developed by the Institute
for Nutraceutical Advancement (INA), was reviewed, optimized, and validated for the detection and quantification of these
phenolic compounds in Echinacea roots and aerial parts. |
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Keywords: | |
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