Analytical use of a biochemical luminous membrane |
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| Authors: | Yoshihito Ikariyama Masuo Aizawa Shuichi Suzuki |
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| Affiliation: | 1. Research Laboratory of Resources Utilization, Tokyo Institute of Technology, Nagatsuta, Midori-ku, Yokohama 227, Japan
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| Abstract: | Luminous membranes were prepared by immobilizing peroxidase (POD) to collagen matrix. The POD luminous membrane generated luninescence in the presence of luminol and H2O2, and the peroxide was determined in the concentration range 10-6-10-3 M by following luminescence emitted from the membrane. Glucose was determined using a luminous membrane in which POD and glucose oxidase (GOD) were coimmobilized. The luminous membranes appear to be feasible for the determination of enzyme substrates and enzyme activity. |
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