A generic small-molecule microarray immobilization strategy |
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Authors: | Mariona Vallè s-MiretMark Bradley |
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Affiliation: | EaStChem, School of Chemistry, University of Edinburgh, Joseph Black Building, West Mains Road, EH9 3JJ Edinburgh, United Kingdom |
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Abstract: | Small-molecule microarrays are often limited by the requirement for each compound undergoing immobilization to contain a common functional group or by the need to prepare glass slides containing photo-reactive groups. Herein, we present a generic strategy that allows any compound library to be immobilized. This was achieved by printing a fluorous-tagged photo-reactive 3-aryl-3-trifluoromethyldiazirine, which undergoes non-selective insertion into compounds following UV-activation, onto fluorous-functionalized glass slides. The arrays could be reused following aqueous stripping and re-assessment of the compounds with the same protein or another target of interest. |
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Keywords: | Small-molecule microarrays Photoreactive functional groups Microwave-assisted synthesis Fluorous affinity |
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