A sensitive and efficient method for trace analysis of some phenolic compounds using simultaneous derivatization and air‐assisted liquid–liquid microextraction from human urine and plasma samples followed by gas chromatography–nitrogen phosphorous detection |
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| Authors: | Mir Ali Farajzadeh Mohammad Reza Afshar Mogaddam Ali Akbar Alizadeh Nabil |
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| Affiliation: | 1. Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Tabriz, Iran;2. Food and Drug Laboratories, Tabriz University of Medical Sciences, Tabriz, Iran |
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| Abstract: | In present study, a simultaneous derivatization and air‐assisted liquid–liquid microextraction method combined with gas chromatography–nitrogen phosphorous detection has been developed for the determination of some phenolic compounds in biological samples. The analytes are derivatized and extracted simultaneously by a fast reaction with 1‐flouro‐2,4‐dinitrobenzene under mild conditions. Under optimal conditions low limits of detection in the range of 0.05–0.34 ng mL?1 are achievable. The obtained extraction recoveries are between 84 and 97% and the relative standard deviations are less than 7.2% for intraday (n = 6) and interday (n = 4) precisions. The proposed method was demonstrated to be a simple and efficient method for the analysis of phenols in biological samples. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | phenolic compounds air‐assisted liquid‐liquid microextraction 1‐flouro‐2,4‐dinitrobenzene gas chromatography nitrogen‐phosphorous detector |
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