A combination of solid‐phase extraction and dispersive solid‐phase extraction effectively reduces the matrix interference in liquid chromatography–ultraviolet detection during pyraclostrobin analysis in perilla leaves |
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Authors: | A. M. Abd El‐Aty Da‐I. Jung Md. Humayun Kabir Jeong‐Heui Choi Sung‐Woo Kim So Jeong Im Young‐Jun Lee Ho‐Chul Shin Chan‐Hyeok Kwon Young‐Wook Son Kang‐Bong Lee Jae‐Han Shim |
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Affiliation: | 1. Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, Konkuk University, Seoul, Republic of Korea;2. Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt;3. Biotechnology Research Institute, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong‐dong, Buk‐gu, Gwangju, Republic of Korea;4. Food Safety Division, Ministry of Food and Drug Safety, Cheongju, Republic of Korea |
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Abstract: | Perilla leaves contain many interfering substances; thus, it is difficult to protect the analytes during identification and integration. Furthermore, increasing the amount of sample to lower the detection limit worsens the situation. To overcome this problem, we established a new method using a combination of solid‐phase extraction and dispersive solid‐phase extraction to analyze pyraclostrobin in perilla leaves by liquid chromatography with ultraviolet absorbance detection. The target compound was quantitated by external calibration with a good determination coefficient (R2 = 0.997). The method was validated (in triplicate) with three fortification levels, and 79.06– 89.10% of the target compound was recovered with a relative standard deviation <4. The limits of detection and quantification were 0.0033 and 0.01 mg/kg, respectively. The method was successfully applied to field samples collected from two different areas at Gwangju and Muan. The decline in the resiudue concentrations was best ascribed to a first‐order kinetic model with half‐lives of 5.7 and 4.6 days. The variation between the patterns was attributed to humidity. Copyright © 2015 John Wiley & Sons, Ltd. |
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Keywords: | pyraclostrobin perilla leaf LC‐UVD interferences SPE‐d‐SPE combination |
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