A rapid and sensitive LC‐MS/MS method for quantification of 3,29‐dibenzoyl rarounitriol in rat plasma: application to a pharmacokinetic study |
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Authors: | Bin Chen Youxin Song Naiqiang Zhu Long Zhao Changjian Liu Xiangwei Meng |
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Institution: | 1. Department of Orthopedics, the Affiliated Hospital of Chengde Medical College, Chengde, China;2. Department of Orthopedics, the First Affiliated Hospital of Dalian Medical University, Dalian, China;3. Gastrointestinal Medicine, the First Hospital of Jilin University, Changchun, China |
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Abstract: | A rapid, sensitive and high‐throughput liquid chromatography–tandem mass spectrometry was established and validated to assay the concentrations of 3,29‐dibenzoyl rarounitriol in rat plasma. Plasma samples were processed by liquid–liquid extraction with ethyl acetate and separated on a Hypersil Gold C18 column (50 × 4.6 mm, 3 µm) at an isocratic flow rate of 0.5 mL/min using methanol–10 mm ammonium acetate–formic acid (90:10:0.1, v/v/v) as mobile phase. The total run time was 5 min for each sample. MS/MS detection was accomplished in selected reaction monitoring mode with positive electrospray ionization. The calibration curve was linear over the concentration range of 0.125–50 ng/mL with lower limit of quantification of 0.125 ng/mL. The intra‐ and inter‐day precisions were <10.1% in terms of coefficient of variation, and the accuracy was within ±11.7% in terms of relative error. The developed method was successfully applied to a pharmacokinetic study of 3,29‐dibenzoyl rarounitriol following intragastric administration of 3.65 mg/kg to Wistar rats. Copyright © 2015 John Wiley & Sons, Ltd. |
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Keywords: | Trichosanthes kirilowii 3 29‐dibenzoyl rarounitriol multiflorane triterpene esters rat plasma pharmacokinetics |
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