Simultaneous determination of ascaridole,p‐cymene and α‐terpinene in rat plasma after oral administration of Chenopodium ambrosioides L. by GC‐MS |
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| Authors: | Xiaoqian Hu Yang Chu Gang Ma Wei Li Xiangyang Wang Hongmei Mo Qihui Yin Jiahua Guo Xiaohui Ma Shuiping Zhou |
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| Institution: | 1. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, Key Laboratory of Analytical Science and Technology of Hebei Province, College of Chemistry and Environmental Science, Hebei University, Baoding, China;2. Tasly Academy, Tasly Holding Group Co. Ltd, Tianjin, China |
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| Abstract: | A sensitive and reliable GC‐MS method was developed and validated for the simultaneous determination of ascaridole, p‐cymene and α‐terpinene in rat plasma using naphthalene as internal standard. The plasma samples were extracted with ethyl acetate. Chromatographic separation was carried out on a HP‐5MS capillary analytical column (30 m × 0.25 mm, 0.25 µm) and detection was performed on a quadrupole mass spectrometer detector operated under selected ion monitoring mode. The method showed excellent linearity over the investigated concentration range (r > 0.99) with the limit of quantitation down to 50, 10 and 5 ng/mL for ascaridole, p‐cymene and α‐terpinene, respectively. The intra‐day and inter‐day precisions (RSD) were <11.3%, and the accuracy was between 90.7 and 113.8%. The method was successfully applied to investigate the pharmacokinetics of Chenopodium ambrosioides L. following oral administration to rats. Copyright © 2015 John Wiley & Sons, Ltd. |
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| Keywords: | ascaridole p‐cymene α ‐terpinene GC‐MS pharmacokinetic study |
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