Preparation of Surface-enhanced Raman Scattering(SERS)-active Optical Fiber Sensor by Laser-induced Ag Deposition and Its Application in Bioidentification of Biotin/Avidin |
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| Authors: | LIU Chunyu WANG Shaoyan FU Cuicui LI Haibo XU Shuping XU Weiqing |
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| Institution: | 1. State Key Laboratory of Supramolecular Structure and Materials, Institute of Theoretical Chemistry, Jilin University, Changchun 130012, P. R. China;
2. College of Science, Changchun University of Science and Technology, Changchun 130022, P. R. China |
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| Abstract: | A surface-enhanced Raman scattering(SERS) optical fiber sensor was prepared by the laser-induced deposition of Ag nanoparticle membrane on a silica optical fiber tip, which was applied to the real time SERS spectral monitoring on the biorecognition of biotin/avidin. The bioidentification of biotin/avidin was carried out through a indirect method, in which the bioidentification is based on the SERS response signal of a labeled dye(Atto610) after its fluorescence has been quenched totally by the deposited Ag nanoparticle membrane. By SERS monitoring the bioidentification process of biotin/avidin, it has been found that this recognition process is finished in 40 min. The lowest detection concentration of biotin is 1.0×10-7 mg/mL. This research is promising in the application of immunoassays on line and in vivo. |
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| Keywords: | Process monitoring Photochemical method Laser-inducement SERS-active optical fiber Biotin/avidin Specific identification |
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