| SYNTHESIS AND SECRETION OF HUMAN ATRIAL NATRIURETIC PEPTIDE IN Saccharomyces cerevisiae |
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| 作者姓名： | 秦宁 魏楠 姚锡惠 李育阳 |
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| 作者单位： | Institute of Genetics，Fudan University，Shanghai 200433，PRC，Institute of Genetics，Fudan University，Shanghai 200433，PRC，Institute of Genetics，Fudan University，Shanghai 200433，PRC，Institute of Genetics，Fudan University，Shanghai 200433，PRC |
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| 摘 要： | A chemically synthesized α-hANP gene was inserted into plasmid YFD18, which was an expression-secretion vector of yeast. The recombinant then transformed in the yeast Y33. The expression level of yeast transformants was about 700 μg ANP/L detected by RIA. More than 99% of expression products were secreted in the culture medium. N-terminal analysis of purified product showed that the first 4 amino acid residues of α-hANP were deleted.
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SYNTHESIS AND SECRETION OF HUMAN ATRIAL NATRIURETIC PEPTIDE IN Saccharomyces cerevisiae |
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| QIN NING WEI NAN YAO XI-HUI AND LI YU-YANG.SYNTHESIS AND SECRETION OF HUMAN ATRIAL NATRIURETIC PEPTIDE IN Saccharomyces cerevisiae[J].Science in China(Chemistry),1991(12). |
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| Authors: | QIN NING WEI NAN YAO XI-HUI AND LI YU-YANG |
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| Abstract: | |
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| Keywords: | human atrial natriuretic peptide Saccharomyces cerevisiae synthesis secretion |
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