Observation of CO(2) in Fourier transform infrared spectral measurements of living Acholeplasma laidlawii cells |
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Authors: | Omura Yoko Okazaki Norio |
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Institution: | Department of Chemistry, Kanagawa Prefectural College of Nursing and Medical Technology, 1-5-1 Nakao, Asahi-ku, Yokohama 241-0815, Japan. y.omura@nifty.com |
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Abstract: | In monitoring the time course of conformational disorder by Fourier transform infrared spectroscopy for intact Acholeplasma laidlawii cells grown at 37 degrees C on binary fatty acid mixtures containing oleic acid and for cells grown on pure palmitic acid, an absorption band at 2343 cm(-1) was observed. The band intensity was found to increase with time. This band was not observed in the spectra for isolated membranes. It is suggested that the 2343 cm(-1) band is due to CO(2) dissolved in water, most likely produced at the final point of fermentation of amino acid by this microorganism. |
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