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A Spatially Programmable DNA Nanorobot Arm to Modulate Anisotropic Gold Nanoparticle Assembly by Enzymatic Excision |
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| Authors: | Prof Jing Yang Yuan Liang Xiang Li Yongpeng Zhang Prof Long Qian Prof Yonggang Ke Prof Cheng Zhang |
| Institution: | 1. School of Computer Science, Key Lab of High Confidence Software Technologies, Peking University, Beijing, 100871 China;2. School of Computer Science, Key Lab of High Confidence Software Technologies, Peking University, Beijing, 100871 China
School of Control and Computer Engineering, North China Electric Power University, Beijing, 102206 China Center for Quantitative Biology, Peking University, Beijing, 100871 China These authors contributed equally to this work.;3. School of Control and Computer Engineering, North China Electric Power University, Beijing, 102206 China;4. Center for Quantitative Biology, Peking University, Beijing, 100871 China;5. Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Emory University School of Medicine, Atlanta, GA 30322 USA |
| Abstract: | Programmable assembly of gold nanoparticle superstructures with precise spatial arrangement has drawn much attention for their unique characteristics in plasmonics and biomedicine. Bio-inspired methods have already provided programmable, molecular approaches to direct AuNP assemblies using biopolymers. The existing methods, however, predominantly use DNA as scaffolds to directly guide the AuNP interactions to produce intended superstructures. New paradigms for regulating AuNP assembly will greatly enrich the toolbox for DNA-directed AuNP manipulation and fabrication. Here, we developed a strategy of using a spatially programmable enzymatic nanorobot arm to modulate anisotropic DNA surface modifications and assembly of AuNPs. Through spatial controls of the proximity of the reactants, the locations of the modifications were precisely regulated. We demonstrated the control of the modifications on a single 15 nm AuNP, as well as on a rectangular DNA origami platform, to direct unique anisotropic AuNP assemblies. This method adds an alternative enzymatic manipulation to DNA-directed AuNP superstructure assembly. |
| Keywords: | AuNPs Assembly DNA Origami Enzymatic Excision Reactions Molecular Spatial Programmability Nanorobot |