A Pro-Fluorescent Ubiquitin-Based Probe to Monitor Cysteine-Based E3 Ligase Activity |
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Authors: | David A Pérez Berrocal Dr Thimmalapura M Vishwanatha Dr Daniel Horn-Ghetko J Josephine Botsch Laura A Hehl Dr Sebastian Kostrhon Dr Mohit Misra Prof?Dr Ivan Ðiki? Dr Paul P Geurink Dr Hans van Dam Prof?Dr Brenda A Schulman Dr Monique P C Mulder |
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Institution: | 1. Department of Cell and Chemical Biology, Leiden University Medical Center (LUMC), Einthovenweg 20, 2333ZC Leiden, The Netherlands;2. Department of Molecular Machines and Signaling, Max Planck Institute of Biochemistry, Am Klopferspitz 18, 82152 Martinsried, Germany;3. Institute of Biochemistry II, Faculty of Medicine, Goethe University, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany |
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Abstract: | Protein post-translational modification with ubiquitin (Ub) is a versatile signal regulating almost all aspects of cell biology, and an increasing range of diseases is associated with impaired Ub modification. In this light, the Ub system offers an attractive, yet underexplored route to the development of novel targeted treatments. A promising strategy for small molecule intervention is posed by the final components of the enzymatic ubiquitination cascade, E3 ligases, as they determine the specificity of the protein ubiquitination pathway. Here, we present UbSRhodol, an autoimmolative Ub-based probe, which upon E3 processing liberates the pro-fluorescent dye, amenable to profile the E3 transthiolation activity for recombinant and in cell-extract E3 ligases. UbSRhodol enabled detection of changes in transthiolation efficacy evoked by enzyme key point mutations or conformational changes, and offers an excellent assay reagent amenable to a high-throughput screening setup allowing the identification of small molecules modulating E3 activity. |
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Keywords: | Biological Activity Drug Discovery Fluorescent Probes E3-Ligases Profiling Transthiolation Activity |
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