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Strong Protein Adhesives through Lanthanide-enhanced Structure Folding and Stack Density |
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| Authors: | Dr Jing Chen Dr Weiwei Shi Dr Yubin Ren Dr Kelu Zhao Dr Yangyi Liu Bo Jia Lai Zhao Ming Li Dr Yawei Liu Dr Juanjuan Su Dr Chao Ma Dr Fan Wang Dr Jing Sun Prof Dr Yang Tian Dr Jingjing Li Prof Dr Hongjie Zhang Prof Dr Kai Liu |
| Institution: | 1. Engineering Research Center of Advanced Rare Earth Materials, Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084 China
State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China National Engineering Laboratory for AIDS Vaccine, School of Life Sciences, Jilin University, Changchun, 130012 China These authors contributed equally to this work.;2. Engineering Research Center of Advanced Rare Earth Materials, Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084 China These authors contributed equally to this work.;3. Engineering Research Center of Advanced Rare Earth Materials, Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084 China;4. State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China;5. Engineering Research Center of Advanced Rare Earth Materials, Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084 China College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049 China;6. College of Materials Science and Opto-Electronic Technology, University of Chinese Academy of Sciences, Beijing, 100049 China;7. School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200241 China;8. Engineering Research Center of Advanced Rare Earth Materials, Ministry of Education), Department of Chemistry, Tsinghua University, Beijing, 100084 China State Key Laboratory of Rare Earth Resource Utilization, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022 China Xiangfu Laboratory, Jiaxing, 314102 China |
| Abstract: | Generating strong adhesion by engineered proteins has the potential for high technical applications. Current studies of adhesive proteins are primarily limited to marine organisms, e.g., mussel adhesive proteins. Here, we present a modular engineering strategy to generate a type of exotic protein adhesives with super strong adhesion behaviors. In the protein complexes, the lanmodulin (LanM) underwent α-helical conformational transition induced by lanthanides, thereby enhancing the stacking density and molecular interactions of adhesive protein. The resulting adhesives exhibited outstanding lap-shear strength of ≈31.7 MPa, surpassing many supramolecular and polymer adhesives. The extreme temperature (−196 to 200 °C) resistance capacity and underwater adhesion performance can significantly broaden their practical application scenarios. Ex vivo and in vivo experiments further demonstrated the persistent adhesion performance for surgical sealing and healing applications. |
| Keywords: | Biomedical Applications Coacervate Adhesives Modular Protein Engineering Molecular Interactions Structural Proteins |