Imaging Hydrogen Sulfide in Hypoxic Tissue with [99mTc]Tc-Gluconate |
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| Authors: | Yongkyoung Kweon Ji-Yong Park Young-Joo Kim Yun-Sang Lee Jae-Min Jeong |
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| Institution: | 1.Department of Nuclear Medicine, Seoul National University College of Medicine, Seoul 03080, Korea; (Y.K.); (J.-Y.P.); (Y.-J.K.); (Y.-S.L.);2.Department of Biomedical Sciences, Seoul National University College of Medicine, Seoul 03080, Korea;3.Department of Nuclear Medicine, Cancer Research Institute, Seoul National University, Seoul 03080, Korea |
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| Abstract: | Hydrogen sulfide (H2S) is the third gasotransmitter and is generated endogenously in hypoxic or inflammatory tissues and various cancers. We have recently demonstrated that endogenous H2S can be imaged with 99mTc]Tc-gluconate. In the present study, we detected H2S generated in hypoxic tissue, both in vitro and in vivo, using 99mTc]Tc-gluconate. In vitro uptake of 99mTc]Tc-gluconate was measured under hypoxic and normoxic conditions, using the colon carcinoma cell line CT26, and was higher in hypoxic cells than that in normoxic cells. An acute hindlimb ischemia-reperfusion model was established in BALB/c mice by exposing the animals to 3 h of ischemia and 3 h of reperfusion prior to in vivo imaging. 99mTc]Tc-gluconate (12.5 MBq) was intravenously injected through the tail vein, and uptake in the lower limb was analyzed by single-photon emission computed tomography/computed tomography (SPECT/CT). SPECT/CT images showed five times higher uptake in the ischemic limb than that in the normal limb. The standard uptake value (SUVmean) of the ischemic limb was 0.39 ± 0.03, while that of the normal limb was 0.07 ± 0.01. 99mTc]Tc-gluconate is a novel imaging agent that can be used both in vitro and in vivo for the detection of endogenous H2S generated in hypoxic tissue. |
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| Keywords: | H2S technetium-99m hypoxia gluconate |
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