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Nanoparticle-Decorated Biomimetic Extracellular Matrix for Cell Nanoencapsulation and Regulation |
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| Authors: | Kyungsene Lee Brandon Davis Xuelin Wang Shubham Mirg Connie Wen Dr Lidya Abune Dr Benjamin E Peterson Dr Li Han Haoyang Chen Prof Hongjun Wang Prof Spencer E Szczesny Prof Yuguo Lei Prof Sri-Rajasekhar Kothapalli Prof Yong Wang |
| Institution: | 1. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802 USA
Contribution: Formal analysis (lead), Investigation (lead), Methodology (lead), Validation (lead), Writing - original draft (equal), Writing - review & editing (equal);2. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802 USA Contribution: Formal analysis (supporting), Investigation (supporting), Methodology (supporting), Validation (supporting);3. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802 USA Contribution: Investigation (supporting), Methodology (supporting), Validation (supporting);4. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802 USA Contribution: Methodology (supporting);5. Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA 16802 USA;6. Department of Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030 USA |
| Abstract: | Cell encapsulation has been studied for various applications ranging from cell transplantation to biological production. However, current encapsulation technologies focus on cell protection rather than cell regulation that is essential to most if not all cell-based applications. Here we report a method for cell nanoencapsulation and regulation using an ultrathin biomimetic extracellular matrix as a cell nanocapsule to carry nanoparticles (CN2). This method allows high-capacity nanoparticle retention at the vicinity of cell surfaces. The encapsulated cells maintain high viability and normal metabolism. When gold nanoparticles (AuNPs) are used as a model to decorate the nanocapsule, light irradiation transiently increases the temperature, leading to the activation of the heat shock protein 70 (HSP70) promoter and the regulation of reporter gene expression. As the biomimetic nanocapsule can be decorated with any or multiple NPs, CN2 is a promising platform for advancing cell-based applications. |
| Keywords: | Biomimetic Material Cell Regulation Cell Surface Engineering Encapsulation Nanoparticle |