Amplified fluorescence quenching and biosensor application of a poly (para-phenylene) cationic polyelectrolyte |
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| Authors: | Mauricio R Pinto Chunyan Tan Michael B Ramey John R Reynolds Troy S Bergstedt David G Whitten Kirk S Schanze |
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| Institution: | 1. Department of Chemistry, University of Florida, PO Box 117200, 32611-7200, Gainesville, FL, USA
3. QTL Biosystems, LLC, 87507, Santa Fe, NM, USA
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| Abstract: | The quenching behavior of a water-soluble cationic poly (para-phenylene) bearing quaternized ammonium side groups (P-NEt 3 + ) was studied. P-NEt 3 + is efficiently quenched by sodium anthraquinone-2,6-disulfonate (AQS) and sodium 1,4,5,8-naphthalenediimide-N,N’-bis (methylsulfonate) (NDS) in aqueous solution via a photo-induced electron-transfer mechanism. Absorption spectra of the NDS/P-NEt 3 + ion-pair complex indicated formation of a stable charge-transfer complex in the ground state. A large spectral shift and band broadening occurred during AQS/P-NEt 3 + complex formation, which is believed to arise due to P-NEt 3 + conformational changes induced by hydrophobic interactions. Finally, a protein sensor that relies on the quenching behavior of P-NEt 3 + was designed based on the quencher-tether-ligand (QTL) approach. AQS tethered to biotin (AQS-E-Biotin) was used along with P-NEt 3 + to sense avidin. |
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