蛋白质样品的非竞争性毛细管电泳免疫分析激光诱导荧光检测

以牛血清白蛋白与其单克隆抗体为样品，初步研究了蛋白质的非竞争性毛细管电泳免疫分析方法。混合温育可以在２０ｍｉｎ内达到平衡，电泳分离在１２ｍｉｎ内完成。当示踪物浓度为３２０ｎｍｏｌ／Ｌ时，所得到的标准曲线的线性范围为８￣１５０ｎｍｏｌ／Ｌ，检出限为５ｎｍｏｌ／Ｌ，并考察了缓冲溶液的浓度、ｐＨ、分离电压等因素的影响。

Noncompetitive Capillary Electrophoresis Based Immunoassay for Proteins with Laser Induced Fluorescence Detection

The noncompetitive capillary electrophoresis based immunoassay for proteins using bovine serum albumin (BSA)and its monoclonal antibody (anti-BSA)as a model system were studied.BSA was labeled with fluorescein isothiocyanate and used as a tracer to determine anti-BSA.The binding reaction between antiBSA and the tracer reached equilibrium in less than 20 minutes,and separation between the immunocomplexes and the free tracer was achieved in 12 minutes using capillary zone electrophoresis.The linear dynamic range of the standard curve was 8~150 nmol/L,and the detection limit was 5nmol/L when a tracer concentration of 320 nmol/L was used.Influences of separation conditions such as buffer concentration,pH and separation voltage was also studied.