| 染料壳聚糖微球的制备及其对人血清白蛋白吸附性能研究 |
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| 引用本文： | 张静,张政朴,贺秉坤. 染料壳聚糖微球的制备及其对人血清白蛋白吸附性能研究[J]. 高分子学报, 2006, 0(4): 640-644 |
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| 作者姓名： | 张静 张政朴 贺秉坤 |
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| 作者单位： | 1. 功能高分子材料教育部重点实验室,南开大学高分子化学研究所,天津,300071
2. 华北制药厂新药中心,石家庄,050015 |
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| 摘 要： | 人血清白蛋白(Human Serum Albumin,HSA)是血浆中含量最丰富的蛋白质,约占血浆总蛋白的60%.在人体内,HSA有许多重要的生理功能[1],临床上广泛应用于手术输血和危重病人补液,治疗创伤休克、烧伤、水肿和低白蛋白血症等,而且能增强人体抵抗能力,是迄今为止产量最大、临床用量最大
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| 关 键 词： | 壳聚糖 Cibacron Blue F3GA 染料亲和吸附剂 HSA |
| 收稿时间： | 2005-11-23 |
| 修稿时间： | 2006-01-18 |
THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN |
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| ZHANG Jing,ZHANG Zhengpu,HE Bingkun. THE PREPARATION OF CIBACRON BLUE F3GA ATTACHED CHITOSAN MICROSPHERES AND THEIR ADSORPTION PROPERTIES FOR HUMAN SERUM ALBUMIN[J]. Acta Polymerica Sinica, 2006, 0(4): 640-644 |
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| Authors: | ZHANG Jing ZHANG Zhengpu HE Bingkun |
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| Affiliation: | 1, Key Laboratory of Functional Polymer Materials, Ministry of Education, Institute of Polymer Chemistry, Nankai University, Tianjin 300071;2, Research Center for New Drugs, North China Pharmaceutical Corporation, Shijiazhuang 050015 |
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| Abstract: | A series of aminated chitosan microspheres were prepared from chitosan,and then an affinity dye-ligand,Cibacron Blue F3GA,was covalently coupled.The maximum attachment of cibacron blue F3GA was 494 μmol/g.Albumin adsorption onto cibacron blue F3GA-attached chitosan microspheres was investigated.The human serum albumin (HSA) equilibrium adsorption capacity was 79.8 mg/g (wet beads).The adsorption equilibrium isotherms appeared to follow a typical Langmuir isotherm.The highest desorption ratio (100%) was achieved by using an eluent of 1.0 mol/L NaSCN (pH 8.0).The cibacron blue F3GA-attached chitosan microspheres could be reused without significant decreases in the adsorption capacities. |
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| Keywords: | Chitosan Cibacron blue F3GA Dye-affinity sorbent HSA |
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