Chlamydomonas reinhardtii genetic variants as probes for fluorescence sensing system in detection of pollutants |
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Authors: | V Scognamiglio D Raffi M Lambreva G Rea A Tibuzzi G Pezzotti U Johanningmeier M T Giardi |
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Institution: | 1. Department of Agrofood, CNR, Institute of Crystallography, Rome Research Area, Rome, Italy 2. Biosensor Srl, 00018, Palombara Sabina, Rome, Italy 3. Institute of Plant Physiology, Martin Luther Universit?t Halle Wittenberg, Weinbergweg, 06099, Halle, Germany
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Abstract: | The unicellular green alga Chlamydomonas reinhardtii is employed here for the setup of a biosensor demonstrator based on multibiomediators for the detection of herbicides. The
detection is based on the activity of photosystem II, the multienzymatic chlorophyll–protein complex located in the thylakoid
membrane that catalyzes the light-dependent photosynthetic primary charge separation and the electron transfer chain in cyanobacteria,
algae, and higher plants. Several C. reinhardtii mutants modified on the D1 photosystem II protein are generated by site-directed mutagenesis and experimentally tested for
the development of a biosensor revealing the modification of the fluorescence parameter (1 − V
J) in the presence of herbicides. The A250R, A250L, A251C, and I163N mutants are highly sensitive to the urea and triazine
herbicide classes; the newly generated F255N mutant is shown to be especially resistant to the class of urea. It follows that
the response of the multibiomediators is associated to a particular herbicide subclass and can be useful to monitor several
species of pollutants. |
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Keywords: | Chlamydomonas reinhardtii Site-directed mutagenesis Biomediator Biosensor Pollutants Fluorescence |
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