Simple and rapid determination of piperacillin and ceftazidime in human plasma samples by HPLC |
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Authors: | M A Campanero A M Zamarreño M Simón M C Dios J R Azanza |
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Institution: | (1) Departamento de Farmacología Clínica, Clinica Universitaria de Navarra, Universidad de Navarra, 31008 Pamplona, Spain;(2) Departamento de Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Navarra, 31008 Pamplona, Spain |
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Abstract: | Summary A simple and rapid liquid chromatographic method has been developed for the determination of therapeutic levels of piperacillin
(I) and ceftazidime (II) in human plasma. Plasma and p-propionamidophenol (internal standard) were precipitated with methanol
(I) or 20% trichloroacetic acid (II). The supernatant was analysed on a 5 μm Spherisorb ODS C18 column with acetonitrile-0.05 M phosphate buffer pH 3.8 as mobile phase and ultraviolet detection at 254 nm. The calibration
graph was linear from 10 to 250 μg mL−1, for (I), and from 5 to 200 μg mL−1 for (II). Intra and inter-day CV did no exceed 2.29% for (I), and were 10.76–11.13%–2.00–5.62 for (II) at concentrations
of 10 μg mL−1 and 250 μg mL−1. |
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Keywords: | Column liquid chromatography Piperacillin in human plasma Ceftazidime in human plasma Pharmacokinetics |
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