| 高效液相色谱-串联质谱法同时测定水溶性迷迭香提取物中迷迭香酸、阿魏酸和咖啡酸的含量 |
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| 引用本文： | 许高燕,刘莹雯,银董红.高效液相色谱-串联质谱法同时测定水溶性迷迭香提取物中迷迭香酸、阿魏酸和咖啡酸的含量[J].分析科学学报,2006,22(5):567-569. |
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| 作者姓名： | 许高燕 刘莹雯 银董红 |
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| 作者单位： | 湖南师范大学化学化工学院,长沙,410081 |
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| 摘 要： | 本文建立了一种同时快速测定水溶性迷迭香提取物中迷迭香酸、阿魏酸和咖啡酸含量的高效液相色谱-串联质谱(HPLC/MS/MS)分析方法。以甲醇和0.1%乙酸铵溶液为HPLC的流动相;MS/MS使用多反应监测(MRM)扫描方式,在3 min内可完成迷迭香酸、阿魏酸和咖啡酸三种化合物的分离分析。上述三种分析物在5~500 ng/mL范围内线性良好(r>0.999);检出限均低于5.0 ng/mL。本法操作简便、分析速度快、灵敏度高,可用于水溶性迷迭香提取物中迷迭香酸、阿魏酸和咖啡酸含量的测定。
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| 关 键 词： | 迷迭香酸 阿魏酸 咖啡酸 液相色谱-质谱 迷迭香提取物 |
| 文章编号： | 1006-6144(2006)05-0567-03 |
| 收稿时间： | 2005-07-19 |
| 修稿时间： | 2005-09-28 |
Simultaneous Determination of Rosmarinic Acid,Caffeic Acid and Ferulic Acid in Water-soluble Extract of Rosemary by LC/MS/MS |
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| XU Gao-yan,LIU Ying-wen,YIN Dong-hong.Simultaneous Determination of Rosmarinic Acid,Caffeic Acid and Ferulic Acid in Water-soluble Extract of Rosemary by LC/MS/MS[J].Journal of Analytical Science,2006,22(5):567-569. |
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| Authors: | XU Gao-yan LIU Ying-wen YIN Dong-hong |
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| Institution: | College of Chemistry and Chemical Engineering, Hunan Normal University, Changsha 410081 |
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| Abstract: | A rapid LC/MS/MS method was developed for the simultaneous determination of rosmarinic acid,caffeic acid and ferulic acid.The mobile phase of HPLC was methanol-0.1% ammonium acetate aqueous solution.As to MS/MS,multi-reaction monitoring(MRM) scan was employed,and a complete analysis can finished in three minutes.The calibration curves for three analytes were linear in the range from 5 ng\5mL~(-1) to 500 ng\5mL~(-1).The developed method was rapid,accurate,robust and convenient,and it was applied to the measurement of rosmarinic acid,caffeic acid and ferulic acid concentrations in water-soluble extract of rosemary with satisfactory results. |
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| Keywords: | Rosmarinic acid Caffeic acid Ferulic acid Liquid Chromatography-Mass Spectrometry Water-soluble extract of rosemary |
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